Part:BBa_K2996009

EGFP gene downstream of the synthetic RSRL sequence

The wrong reading frame and existing stop codon at the leader together will insure no expression of the florescent protein in the original situation.

Upon information input, that is addition of corresponding inducer, spacer adaptation from cas1/2 will result in an addition of 61 base pairs, 33bp new spacer and 28bp replicated repeat, into the integration cassette. Stop codon will be moved out of frame, while EGFP in the open reading frame.

They are spaced by BamHⅠrestriction site, due to our clone method. This scar can be random and won’t affect the biological function of EGFP. And this composite part, can be regulated by any promotor as well.

Results are shown on BBa_K2996011 给链接.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 159
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]